| Bouvette J, Korkut DN, Fouillen A, Amellah S, Nanci A, Durocher Y, Omichinski JG, Legault P
(2018)
"High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension."
BMC Biotechnol.
First published online Dec 6, 2018.
|
|
| Dagenais P, Girard N, Bonneau E, Legault P
(2017)
"Insights into RNA structure and dynamics from recent NMR and X-ray studies of the Neurospora Varkud satellite ribozyme."
RNA.
|
|
| Di Tomasso G, Miller Jenkins, LM, Legault P
(2016)
"ARiBo pull-down for riboproteomic studies based on label-free quantitative mass spectrometry."
RNA.
|
|
| Lacroix-Labonté J, Girard N, Dagenais P, Legault P
(2016)
"Rational engineering of the Neurospora VS ribozyme to allow substrate recognition via different kissing-loop interactions."
Nucleic Acids Res.
|
|
| Bonneau E, Girard N, Lemieux S, Legault P
(2015)
"The NMR structure of the II-III-VI three-way junction from the Neurospora VS ribozyme reveals a critical tertiary interaction and provides new insights into the global ribozyme structure."
RNA.
First published online Jun 29, 2015.
|
|
| Di Tomasso G, Salvail-Lacoste A, Bouvette J, Omichinski JG, Legault P
(2014)
"Affinity purification of in vitro transcribed RNA with homogeneous ends using a 3'-ARiBo tag."
Methods Enzymol. 549:49-84.
|
|
| Dieckmann,T., Piazza, M., Bonneau, E. and Legault P
(2014)
"Biomolecular NMR spectroscopy of ribonucleic acids."
In: Encyclopedia of Life Sciences, John Wiley & Sons, Ltd: Chichester http://www.els.net/.
Posted on line July 15, 2014 [DOI:10.1002/9780470015902.a0021033.pub2]
|
|
| Bonneau E, Legault P
(2014)
"Nuclear magnetic resonance structure of the III-IV-V three-way junction from the Varkud satellite ribozyme and identification of magnesium-binding sites using paramagnetic relaxation enhancement."
Biochemistry. 53(39):6264-75.
First published online Sept 19, 2014.
|
|
| Bouchard P, Legault P
(2014)
"A remarkably stable kissing-loop interaction defines substrate recognition by the Neurospora Varkud Satellite ribozyme."
RNA. 20(9):1451-64.
First published online Jul 22, 2014.
|
|
| Desjardins A, Bouvette J, Legault P
(2014)
"Stepwise assembly of multiple Lin28 proteins on the terminal loop of let-7 miRNA precursors."
Nucleic Acids Res. 42(7):4615-28.
First published online Jan 21, 2014.
|
|
| Bonneau E, Legault P
(2014)
"NMR localization of divalent cations at the active site of the Neurospora VS ribozyme provides insights into RNA-metal-ion interactions."
Biochemistry. 53(3):579-90.
First published online Jan 10, 2014.
|
|
| Bouchard P, Legault P
(2014)
"Structural insights into substrate recognition by the Neurospora Varkud satellite ribozyme: importance of U-turns at the kissing-loop junction."
Biochemistry. 53(1):258-69.
First published online Dec 17, 2013.
|
|
| Raiola L, Lussier-Price M, Gagnon D, Lafrance-Vanasse J, Mascle X, Arseneault G, Legault P, Archambault J, Omichinski JG
(2013)
"Structural characterization
of a noncovalent complex between ubiquitin and the transactivation
domain of the erythroid-specific factor EKLF"
Structure. 21(11):2014-24.
First published online Oct 17, 2013.
|
|
| Salvail-Lacoste, A, Di Tomasso G, Piette, BL and
Legault, P (2013) "Affinity Purification
of T7 RNA Transcripts with Homogenous Ends Using ARiBo and
CRISPR Tags" RNA. 19(7):1003-1014. First published online
May 8, 2013. |
|
| Lafrance-Vanasse, J, Arseneault, G, Cappadocia, L,
Legault, P and Omichinski, JG (2013)
"Structural and Functional Evidence that Rad4 Competes
with Rad2 for Binding to the Tfb1 Subunit of TFIIH in NER"
Nucleic Acids Res. 41(4): 2736-45. First published on line
January 7, 2013. |
|
| DiTomasso, G. and Legault, P (2012)
"Nouvelle méthode de purification d'ARN par
affinité avec l'étiquette ARiBo" Médecine Sciences Amériques.
First published online April 15, 2012 |
|
| Dagenais, P and Legault, P (2012)
"Preparative Separation of Ribonucleoside Monophosphates
by Ion-Pair Reverse-Phase HPLC" Methods in Molecular
Biology. Vol. 941 (Recombinant and In Vitro RNA Synthesis):
247-256. |
|
| Di Tomasso, G, Dagenais, P, Desjardins, A,
Rompré-Brodeur, A, Delfosse, V and Legault,
P (2012) "Affinity Purification of RNA Using an
ARiBo Tag" Methods in Molecular Biology. Vol. 941
(Recombinant and In Vitro RNA Synthesis): 137-155. |
|
| Di Tomasso, G, Lampron, P, Omichinski, JG and
Legault, P (2012) "Preparation of the
λNpeptide-GST Fusion Protein for Affinity Immobilization
of RNA" Methods in Molecular Biology. Vol. 941
(Recombinant and In Vitro RNA Synthesis): 123-135. |
|
| Lafrance-Vanasse J, Arseneault G, Cappadocia L, Chen H-T,
Legault, P and Omichinski JG (2012)
"Structural and Functional Characterization of
Interactions Involving the Tfb1 Subunit of TFIIH and the NER
Factor Rad2" Nucleic Acids Res. 40(12): 5739-5750. First
published online February 28, 2012 DOI:10.1093/nar/gks194. |
|
| Langlois, C, Del Gatto, A, Arseneault, G, Lafrance-Vanasse,
J, De Simone, M, Morse, T, de Paola, I, Lussier-Price, M,
Legault, P, Pedone, C, Zaccaro, L, Omichinski,
J (2012) "Structure-Based Design of a Potent Artificial
Transactivation Domain Based on p53" J. Am. Chem. Soc. 134
(3): 1715-1723. First published online December 19, 2011
DOI:10.1021/ja208999e. |
|
| Lacroix-Labonté, J, Girard, N, Lemieux, S and
Legault, P (2012) "Helix-length
compensation studies reveal the adaptability of the VS ribozyme
architecture" Nucleic Acids Res. 40(5): 2312-2329. First
published online November 15, 2011
DOI:10.1093/nar/gkr1018. |
|
| Desjardins, A, Yang, A, Bouvette, J, Omichinski, JG and
Legault, P (2012) "Importance of the
NCp7-like domain in the recognition of the let-7g precursor
miRNA by the pluripotency factor Lin28" Nucleic Acids Res.
40(4): 1767-1777. First published online October 19, 2011
DOI:10.1093/nar/gkr808. |
|
| Tremblay, R, Lemay, JF, Blouin, S, Mulhbacher, J, Bonneau,
E, Legault, P, Dupont, P, Penedo, JC,
Lafontaine, DA (2011) "Constitutive regulatory activity of
an evolutionary-excluded riboswitch variant" J. Biol.
Chem. 2011 286(31): 27406-15. |
|
| Desjardins G, Bonneau E, Girard, N, Boisbouvier, J and
Legault P (2011) "NMR structure of the
A730 loop of the Neurospora VS ribozyme; insights into the
formation of the active site" Nucleic Acids Res. 2011 Jan
25. DOI: 10.1093/nar/gkq1244. |
|
| Di Tomasso G, Lampron P, Dagenais P, Omichinski JG and
Legault P (2010) "The ARiBo tag: a
reliable tool for affinity purification of RNAs under native
conditions." Nucleic Acids Res. 2010 Nov 11. DOI:
10.1093/nar/gkq1084. |
|
| Delfosse V, Bouchard P, Bonneau E, Dagenais P, Lemay JF,
Lafontaine DA and Legault P (2010) "
Riboswitch structure: an internal residue mimicking the purine
ligand." Nucleic Acids Res.;38(6):2057-68. Epub 2009 Dec
18. DOI: 10.1093/nar/gkp1080. |
|
| Wang D, Iera J, Baker H, Hogan P, Ptak R, Yang L, Hartman
T, Buckheit RW Jr, Desjardins A, Yang A, Legault
P, Yedavalli V, Jeang KT and Appella DH. (2009)
"Multivalent binding oligomers inhibit HIV Tat-TAR
interaction critical for viral replication." Bioorg Med
Chem Lett. ;19(24):6893-7. Epub 2009 Oct 23.
DOI:10.1016/j.bmcl.2009.10.078. |
|
| Dikeakos, J.D, Di Lello, P., Lacombe, M.-J., Ghirlando, R.,
Legault, P., Reudelhuber, T. L. and
Omichinski, J.G (2009) "Functional and structural
characterization of a dense core secretory granule sorting
domain from the PC1/3 protease" Proc. Natl. Acad. Sci.
U.S.A. 48; 7408-7413. Publication Date (Web): April 17, 2009.
DOI: 10.1073/pnas.0809576106. |
|
| Yang, A., Abbott, K.A, Desjardins, A., Di Lello, P.,
Omichinski, J.G. and Legault, P. (2009)
"NMR structure of a complex formed by the
carboxyl-terminal domain of human RAP74 and a phosphorylated
peptide from the central domain of the FCP1 phosphatase"
Biochemistry. 48 (9), pp 19641974. Publication Date (Web):
February 12, 2009. DOI: 10.1021/bi801549m. |
|
| Dieckmann T. and Legault P. (April 2008)
"Biomolecular NMR spectroscopy of ribonucleic acids"
In : Encyclopedia of Life Sciences, John Wiley & Sons, Ltd:
Chichester http://www.els.net/. |
|
| Langlois, C., Mas, C., Di Lello, P., Miller Jenkins, L. M.,
Legault, P. and Omichinski, J.G. (2008)
"NMR structure of the complex between the Tfb1 subunit of
TFIIH and the activation domain of VP16: Structural
similarities between VP16 and p53" J. Am. Chem. Soc.
130(32); 10596-10604. DOI:
10.1021/ja800975h. |
|
| Bouchard, P., Lacroix-Labonté, J., Desjardins, G.,
Lampron, P., Lemieux, S., Major, F. and Legault,
P. (2008) "Role of SLV in SLI recognition by the
Neurospora VS ribozyme" RNA 14; 736-748.
Published in Advance February 26, 2008,
10.1261/rna.824308. |
|
| Di Lello, P., Miller Jenkins, L. M., Mas, C., Langlois, C.,
Malitskaya, E., Fradette-Turcotte, A., Archambault, J.,
Legault, P., and Omichinski, J. G (2008)
"p53 and TFIIEalpha share a common binding site on the
Tfb1/p62 subunit of TFIIH" Proc. Natl. Acad. Sci. U.S.A.
105; 106-111. |
|
| Miller-Jenkins, L. M., Durell, S. R., Maynard, A.T., Stahl,
S. J., Inman, J. K., Appella, E., Legault, P.
and Omichinski, J. G. (2006) "Comparison of the
specificity of interaction of cellular and viral zinc binding
domains with 2-mercaptobenzamidine thioesters." J. Am.
Chem. Soc. 128; 11964-11976. |
|
| Campbell, D. O., Bouchard, P., Desjardins, G. and
Legault, P. (2006) "NMR structure of the
Varkud satellite ribozyme stem-loop V in the presence of
magnesium ions and localization of metal binding sites"
Biochemistry 45; 10591-10605. |
|
| Di Lello, P., Miller Jenkins, Lisa M., Jones, T. N.,
Nguyen, B. D., Hara, T., Yamaguchi, H., Dikeakos, J. D.,
Appella, E., Legault, P. and Omichinski, J. G.
(2006) "Structure of the Tfb1/p53 complex : Insights into
the interaction between the p62/Tfb1 subunit of TFIIH and the
activation domain of p53. " Molecular Cell
22; 731-740. |
|
| Di Lello, P., Nguyen, B. D., Jones, T. N., Potempa, K.,
Kobor, M. S., Legault, P. and Omichinski, J.
G. (2005) "NMR structure of the amino-terminal domain from
the Tfb1 subunit of TFIIH and characterization of its
phosphoinosititde and VP16 binding sites"
Biochemistry 44; 7678-7686. |
|
| Miller-Jenkins, L. M., Byrd, J. C., Srivastava, P., Stahl,
S. J., Inman, K., Appella, E., Omichinski, J. G. and
Legault, P. (2005) "Characterization of
the mechanism of thioster compound inactivation of the HIV-1
nucleocapsid protein NCp7" J. Med. Chem.
48; 2847-2858. |
|
| Campbell, D. O. and Legault, P. (2005)
"NMR structure of the VS ribozyme stem-loop V RNA and
magnesium-ion binding from chemical-shift mapping"
Biochemistry 44; 4157-4170 |
|
| Abbott, K. L., Renfrow, M. B., Chalmers, M. J., Nguyen,
B. D., Marshall, A. G., Legault, P. and
Omichinski, J. G. (2005) "Enhanced binding of RNAPII
CTP phosphatase FCP1 to RAP74 following CK2
phosphorylation" Biochemistry
44; 2732-2745. |
|
| Abbott, K. L., Archambault, J., Xiao, H., Nguyen, B.
D., Roeder, R. G., Greenblatt, J., Omichinski, J. G. and
Legault, P. (2005) "Interactions of
the HIV-1 tat and RAP74 proteins with the RNAPII CTD
phosphatase FCP1" Biochemistry
44; 2716-2731. |
|
| Nguyen, B. D., Di Lello, P., Legault,
P. and Omichinski, J. G. (2004) "Letter to
the Editor: 1H, 15N, and 13C resonance assignment of the
amino-terminal domain of the Tfb1 subunit of yeast
TFIIH" J. Biomol. NMR 31;
173-174. |
|
| Benison, G., Di Lello, P., Shokes, J. E., Cosper, N.
J., Scott, R. A., Legault, P., Omichinski,
J. G. (2004) "A stable mercury-containing complex of
the organomercurial lyase MerB: catalysis, product Release,
and direct transfer to MerA" Biochemistry
43; 8333-8345. |
|
| Di Lello, P., Benison, G. C., Valafar, H., Pitts, K.
E., Summers, A. O., Legault, P. and
Omichinski, J. G. (2004) "NMR structural studies
reveal a novel protein fold for MerB, the organomercurial
lyase involved in the bacterial mercury resistance
system" Biochemistry 43;
8322-8332. |
|
| Di Lello, P., Benison, G. C., Omichinski, J. G., and
Legault, P. (2004) "Letter to the
Editor: 1H, 15N, and 13C resonance assignment of the 23 kDa
organomercurial lyase MerB in its free and mercury-bound
forms" J. Biomol. NMR 29;
457-458. |
N/A
|
| Hoffmann, B., Mitchell, G. T., Gendron, P., Major, F.,
Andersen, A. A. Collins, R. A., and Legault,
P. (2003) "NMR structure of the active
conformation of the Varkud satellite ribozyme cleavage
site" Proc. Natl. Acad. Sci. U.S.A.
100(12); 7003-7008. |
|
| Nguyen, B. D., Abbott, K., Potempa, K. Kobor, M. S.,
Archambault, J., Greenblatt, J., Legault,
P. Omichinski, J. G. (2003) "NMR structure of
a complex containing the TFIIF subunit RAP74 and the RNA
polymerase II carboxyl-terminal domain phosphatase
FCP1" Proc. Natl. Acad. Sci. U.S.A.
100(10); 5688-5693. |
|
| Nguyen, B. D., Chen, H.-T., Kobor, M., Grzesiek, S.,
Greenblatt, J., Legault, P., and
Omichinski, J. G. (2003) "Solution Structure of the
carboxyl-terminal domain of RAP74 and NMR characterization
of the FCP1-binding sites of RAP74 and human TFIIB"
Biochemistry, 42(6);
1460-1469. |
|
| Chen, H.-T., Legault, P., Glushka, J.,
Omichinski, J. G., Scott, R. A. (2000) "Structure of a
(cys3his) zinc ribbon, a ubiquitous motif in archaeal and
eucaryal transcription" Protein Science.
9; 1743-1752. |
|
| Hoogstraten, C. G., Legault, P., and
Pardi, A. (1998) "NMR solution structure of the
lead-dependent ribozyme: evidence for dynamics in RNA
catalysis." J. Mol. Biol.
284; 337-350. |
|
| Legault, P., Hoogstraten, C. G.,
Metlitzky, E., Pardi, A. (1998) "Order, dynamics and
metal binding in the lead-dependent ribozyme." J.
Mol. Biol. 284; 325-335. |
|
| Greenblatt, J., Mah, T.-F., Legault,
P., Mogridge, J., Li, J., Kay, L.E. (1998)
"Structure and mechanism in transcriptional
antitermination by the bacteriophage lambda N
protein." Cold Spring Harbor symposia on
quantitative biology Vol. 63
(Mechanisms of Transcription) Cold Spring Harbor
Laboratory. New York, Pp. 327-336. |
N/A
|
| Legault, P., Li, J., Mogridge, J.,
Kay, L. E., Greenblatt, J.(1998) "NMR structure of the
bacteriophage lambda N-Peptide /BoxB RNA complex:
recognition of a GNRA fold by an arginine-rich motif."
Cell 93; 289-299. |
|
| Simorre, J.-P., Legault, P., Baidya,
N., Uhlenbeck, O. C., Maloney, L., Wincott, F., Usman, N.,
Beigelman, L., Pardi, A. (1998) "Structural variation
induced by different nucleotides at the cleavage site of
the hammerhead ribozyme." Biochemistry
37; 4034-4044. |
|
| Mogridge, J., Legault, P., Li, J., Van
Oene, M. D., Kay, L. E., Greenblatt, J. (1998)
"Independent ligand-induced folding of the RNA-binding
domain and two functionally distinct antitermination
regions in the phage lambda N protein." Molec.
Cell. 1;
265-275. |
|
| Zwahlen, C.*, Legault, P.*, Vincent,
S. J. F., Greenblatt, J., Konrat, R. and Kay, L. E. (1997)
"Methods for measurement of intermolecular NOEs by
multinuclear NMR spectroscopy: Application to a
bacteriophage lambda N-peptide/boxB RNA complex."
J. Am. Chem. Soc. 119;
6711-6721.(* = Equal contribution) |
N/A
|
| Legault, P. and Pardi, A. (1997)
"Unusual dynamics and pKa shift at the active site of
a lead-dependent ribozyme." J. Am. Chem. Soc.
119; 6621-6628. |
N/A
|
| Simorre, J.-P., Legault, P., Hangar,
A. B., Michiels, P., and Pardi, A. (1997) "A
conformational change in the catalytic core of the
hammerhead ribozyme upon cleavage of an RNA
susbstrate." Biochemistry
36; 518-525. |
|
| Rao, N. S., Legault, P., Muhandiram,
D. R., Greenblatt, J., Battiste, J. L., Williamson, J. R.,
and Kay, L. E. (1996) "NMR pulse schemes for the
sequential assignment of arginine sidechain H-epsilon
protons." J. Mag. Res. Series B
113; 272-276. |
N/A
|
| Mueller, L., Legault, P., and Pardi,
A. (1995) "Improved RNA structure determination by
detection of NOE contacts to exchange-broadened amino
protons." J. Am. Chem. Soc.
117;11043-11048. |
N/A
|
| Zimmermann, G. R., Legault, P.,
Selsted, M. E., and Pardi, A. (1995) "Solution
structure of bovine neutrophil beta-defensin-12: The
peptide fold of the beta-defensins is identical to that of
the classical defensins." Biochemistry
34;13663-13671. |
N/A
|
| Legault, P., Jucker, F. M., and Pardi,
A. (1995) "Improved measurement of 13C, 31P J
coupling constants in isotopically labeled RNA."
FEBS Lett. 362; 156-160. |
|
| Legault, P. and Pardi, A. (1994)
"In situ probing of adenine protonation in RNA by
13C NMR." J. Am.
Chem. Soc. 116, 8390-8391. |
N/A
|
| Legault, P., Farmer II, B. T.,
Mueller, L., and Pardi, A. (1994) "Through-bond
correlation of adenine protons in a 13C-labeled ribozyme." J. Am.
Chem. Soc. 116, 2203-2204. |
N/A
|
| Legault, P. and Pardi, A. (1994)
"31P chemical shift as a
probe of structural motifs in RNA." J. Mag.
Res. Series B 103, 82-86. |
N/A
|
| Legault, P., Herschlag, D., Celander,
D. W., and Cech, T. R. (1992) "Mutations at the
guanosine binding site of the Tetrahymena ribozyme
also affect site-specific hydrolysis." Nucleic
Acids Res. 20, 6613-6619.
|
|
| Nikonowicz, E. P., Sirr, A., Legault,
P., Jucker, F. M., Baer, L. M., and Pardi, A.
(1992) "Preparation of 13C and 15N
labelled RNAs for heteronuclear multi-dimensional NMR
studies." Nucleic Acids Res.
20, 4507-4513. |
|
| Amman, H., Boulanger, Y., Legault, P.,
and Vinay, P. (1989) "An incubation system for the NMR
study of kidney tubules." Magn. Res. Med.
12, 339-347. |
N/A
|
| Boulanger, Y., Legault, P., Tejedor,
A., Vinay, P., and Thériault, Y. (1988)
"Biochemical characterization and osmolytes in
papillary collecting ducts from pig and dog kidneys."
Can. J. Physiol. Pharmacol. 66,
1282-1290. |
N/A
|
